|
CABRI:ICLC |
|
Accession_number |
ICLC HTL03004
|
|
Cell_line_name |
B1647
|
|
Brief_description |
Species: human; Tissue: bone marrow; Tumor: leukemia; Derived from: a patient with acute myeloid leukemia with a complete erythro-megakaryocytic phenotype
|
|
Description |
Species: human male 14 year old; Tissue: bone marrow; Tumor: leukemia; Derived from: a patient with acute myeloid leukemia with a complete erythro-megakaryocytic phenotype
|
|
Depositor |
Prof. G.P. Bagnara, Institute of Histology and Embryology, University of Bologna, Italy
|
|
Bibliographic_reference |
Br J Haematol 1997;98:549-559 [PMID: 9332307
] |
|
Morphology |
round cells, erythromegakaryocytic, grown in suspension
|
|
Culture_conditions |
continuous culture; Iscove's modified Dulbecco's medium + 10% FBS + 2mM L-Glutamine ; Split 1:3-1:6; 37C, 5% CO2
|
|
Viruses |
Search for viruses was not performed
|
|
Properties |
Expression of both c-mpl and erythropoietin receptor (Epo-R). Spontaneous B1647 cell proliferation is significantly potentiated by thrombopoietin (TPO)
|
|
Release_conditions |
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
|
|
Hazard |
-
|
|
Passage_number |
>50
|
|
Freezing_medium |
Culture medium + 70% FBS + 10% DMSO
|
|
Sterility |
mycoplasma negative, HOECHST and PCR
|
|
Validation_assays |
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
|
|
Further_bibliography |
Haematologica 2002;87:1242-1247 [PMID: 12495897
]
|